RNA

Part:BBa_K1124009:Design

Designed by: Yuta Otsuka   Group: iGEM13_UT-Tokyo   (2013-09-11)

anti-tyrR sRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was generated by site-directed mutagenesis on MicC-sRNA scaffold(BBa_K1124005), in which the target-binding sequence was added to the scaffold. The target binding sequence was designed complementary to the tyrR gene of E. coli K-12 strain.


Source

We designed oligo DNA complementary to the first 24nt of tyrR CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold (BBa_K1124005) using PCR technique.

References

Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.